Work

Enzymatic Cleavage and Repair of Transforming Dna

Year: 1969

Type: article

Abstract: Single-strand nicks caused by DNase I are capable of inactivating B. subtilis transforming DNA. Under suitable conditions, the polynucleotide joining enzyme from E. coli and a similar DPN-requiring activity from B. subtilis can completely repair this damage, restore biological activity, and increase the single-strand molecular weight. The rates of inactivation of a single genetic marker and of a four-marker linkage group suggest that a single-strand nick is inactivating even when far from the site of genetic damage.

Source: Proceedings of the National Academy of Sciences

DOI: 10.1073/pnas.62.1.289

Authors: P. J. Laipis, B. M. Olivera, A. T. Ganesan

Institutions: Stanford Medicine, Stanford University

Language: English

Topic: Bacterial Genetics and Biotechnology

Subfield: Genetics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

OpenAlex ID: W2056357682

FWCI: 2.769

Cites: 13

Cited by: 30

Related to: 10