Work

Cobra polypeptide cytotoxin I and marine worm polypeptide cytotoxin A‐IV are potent and selective inhibitors of phospholipid‐sensitive Ca2+‐dependent protein kinase

Year: 1983

Type: article

Abstract: The effects of a number of polypeptide cytotoxins and neurotoxins on various protein kinases were examined. It was found that cobra cytotoxin I and marine worm cytotoxin A-IV effectively and specifically inhibited phospholipid-sensitive Ca2+-dependent protein kinase (PL-Ca-PK) relative to myosin light chain kinase and cyclic nucleotide-dependent protein kinases. Inhibition of PL-Ca-PK by these cytotoxins could be overcome by phosphatidylserine. Neurotoxins, in comparison, were much less effective inhibitors. The present findings indicated that these polypeptide cytotoxins, unlike other agents reported to date, were selective inhibitors of PL-Ca-PK and could be used to differentiate Ca2+-dependent events regulated by phospholipid or calmodulin.

Source: FEBS Letters

DOI: 10.1016/0014-5793(83)80144-6

Authors: J.F. Kuo, Robert L. Raynor, Gonzalo J. Mazzei, Randall C. Schatzman, R.Scott Turner, William R. Kem

Institutions: Emory University, University of Florida Health, University of Florida

Language: English

Topic: Protein Kinase Regulation and GTPase Signaling

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: bronze

Funders: National Science Foundation, U.S. Public Health Service

Award: Structure and Function of a Polypeptide Neurotoxin