Work

Electroporation for the efficient transfection of mammalian cells with DNA

Year: 1987

Type: article

Abstract: A simple and reproducible procedure for the introduction of DNA into mammalian cells by electroporation is described. The parameters involving the cells, the DNA, and the electric field are investigated. The procedure has been applied to a broad range of animal cells. It is capable of transforming more than 1% of the viable cells to the stable expression of a selectable marker.

Source: Nucleic Acids Research

DOI: 10.1093/nar/15.3.1311

Authors: Gilbert Chu, Hiroshi Hayakawa, Paul Berg

Institutions: Stanford Medicine, Stanford University

Language: English

Topic: Microbial Inactivation Methods

Subfield: Biotechnology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: bronze

Funders: Jane Coffin Childs Memorial Fund for Medical Research, National Institute of General Medical Sciences

Award: MECHANISMS &REGULATION OF GENE EXPRESSION