Year: 1992
Type: article
Abstract: This article gives a brief overview over the problem of obtaining three-dimensional im- ages of macromolecules that occur in the form of single particles.The method of random-conical data collection and reconstruction, which has been successfully used for a variety of complex macromolec- ular assemblies such as the ribosome and the calcium release channel, receives particular attention.The roles of classification and restoration is obtaining artifact-free reconstructions, as well as the feasi- bility of merging different reconstructions are discussed both for negatively stained and ice-embedded macromolecules.Microsc.Microanal.Microstruct.
Author: Joachim Frank
Institutions: New York State Department of Health, Wadsworth Center
Language: English
Subfield: Structural Biology
Domain: Life Sciences
Open Access status: bronze
Funder: National Institutes of Health
OpenAlex ID: W2057178131