Work

Introduction of an Aliphatic Ketone into Recombinant Proteins in a Bacterial Strain that Overexpresses an Editing‐Impaired Leucyl‐tRNA Synthetase

Year: 2009

Type: article

Abstract: Like leucine? A leucine analogue containing a ketone has been incorporated into proteins in E. coli. Only E. coli strains overexpressing an editing-deficient leucyl-tRNA synthetase were capable of synthesizing proteins with the aliphatic ketone amino acid. Modification of ketone-containing proteins under mild conditions has been demonstrated.

Source: ChemBioChem

DOI: 10.1002/cbic.200900407

Authors: Yi Tang, Pin Wang, James A. Van Deventer, A. James Link, David A. Tirrell

Institutions: University of California, Los Angeles, University of Southern California, California Institute of Technology, Princeton University

Language: English

Topic: RNA and protein synthesis mechanisms

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funder: National Institute of General Medical Sciences

Awards: G3086510201, G6238445359