Year: 2001
Type: article
Abstract: The expression of interphotoreceptor retinoid binding protein (IRBP) is limited to photoreceptor cells of the retina and pinealocytes of the pineal gland. We sought to define cis-elements of the mouse IRBP 5' flanking region that are required for this restricted activity. In vitro transient transfections of retinoblastoma and neuroblastoma cells and in vivo experiments with transgenic Xenopus laevis indicate that -1783/+101 and -156/+101 IRBP gene fragments directed expression predominantly to the retina and pineal, with minor neuronal expression elsewhere. In contrast, a -70/+101 fragment was less restrictive in controlling expression, exhibiting activity not only in retina, but also in forebrain, hindbrain, spinal cord, and motor neurons innervating gills.
Source: FEBS Letters
Authors: J.H. Boatright, B.E. Knox, K.M. Jones, E. Stodulkova, H.T. Nguyen, S.A. Padove, D.E. Borst, J.M. Nickerson
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: bronze
Awards: IRBP GENE EXPRESSION IN THE RETINA, CONTROL OF IRBP GENE EXPRESSION IN THE EYE, Regulation of Rhodopsin Expression, G5520316217, PERMISSIVE RETINAL GENE EXPRESSION VIA HYPOMETHYLATION, P30 Wilmer Core Grant for Vision Research, G8559660125, Molecular Mechanisms of Photoreceptor Function, P30-Core Grant for Vision Research
OpenAlex ID: W2058005281