Towards construction of a high resolution map of the mouse genome using PCR-analysed microsatellites
Year: 1990
Type: article
Abstract: Fifty sequences from the mouse genome database containing simple sequence repeats or microsatellites have been analysed for size variation using the polymerase chain reaction and gel electrophoresis. 88% of the sequences, most of which contain the dinucleotide repeat, CA/GT, showed size variations between different inbred strains of mice and the wild mouse, Mus spretus. 62% of sequences had 3 or more alleles. GA/CT and AT/TA-containing sequences were also variable. About half of these size variants were detectable by agarose gel electrophoresis. This simple approach is extremely useful in linkage and genome mapping studies and will facilitate construction of high resolution maps of both the mouse and human genomes.
Source: Nucleic Acids Research
Authors: Jennifer M. Love, Andrew M. Knight, Marcia A. McAleer, John A. Todd
Institution: John Radcliffe Hospital
Language: English
Subfield: Genetics
Domain: Life Sciences
Open Access status: bronze
Funders: Wellcome Trust, Medical Research Council
OpenAlex ID: W2058201069