Work

SIVQ-LCM Protocol for the ArcturusXT Instrument

Year: 2014

Type: article

Abstract: SIVQ-LCM is a new methodology that automates and streamlines the more traditional, user-dependent laser dissection process. It aims to create an advanced, rapidly customizable laser dissection platform technology. In this report, we describe the integration of the image analysis software Spatially Invariant Vector Quantization (SIVQ) onto the ArcturusXT instrument. The ArcturusXT system contains both an infrared (IR) and ultraviolet (UV) laser, allowing for specific cell or large area dissections. The principal goal is to improve the speed, accuracy, and reproducibility of the laser dissection to increase sample throughput. This novel approach facilitates microdissection of both animal and human tissues in research and clinical workflows.

Source: Journal of Visualized Experiments

DOI: 10.3791/51662

Authors: Jason D. Hipp, Jerome Cheng, Jeffrey C. Hanson, Avi Z. Rosenberg, Michael R. Emmert-Buck, Michael A. Tangrea, Ulysses J. Balis

Institutions: National Institutes of Health, National Cancer Institute, University of Michigan

Language: English

Topic: Cell Image Analysis Techniques

Subfield: Biophysics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: National Institutes of Health, National Cancer Institute

OpenAlex ID: W2058812109

FWCI: 0.373

Cites: 24

Cited by: 6

Related to: 10