Work

Back‐Scattering Interferometry: An Ultrasensitive Method for the Unperturbed Detection of Acetylcholinesterase–Inhibitor Interactions

Year: 2012

Type: article

Abstract: A series of inhibitors of acetylcholinesterase (AChE) have been screened by back-scattering interferometry (BSI). Enzyme levels as low as 100 pM (22,000 molecules of AChE) can be detected. This method can be used to screen for mixed AChE inhibitors, agents that have shown high efficacy against Alzheimer's disease, by detecting dual-binding interactions. E = enzyme, I = inhibitor, S = substrate.

Source: Angewandte Chemie International Edition

DOI: 10.1002/anie.201203640

Authors: Gabrielle L. Haddad, Sherri C. Young, Ned D. Heindel, Darryl J. Bornhop, Robert A. Flowers

Institutions: Lehigh University, Vanderbilt University

Language: English

Topic: Cholinesterase and Neurodegenerative Diseases

Subfield: Pharmacology (medical)

Field: Medicine

Domain: Health Sciences

Open Access status: green

Funder: National Institute of Arthritis and Musculoskeletal and Skin Diseases

Award: Rutgers University CounterACT Center of Excellence