Work

Isolation of the Predominant Cycloartane Glycoside, Sutherlandioside B, from Sutherlandia frutescens (L.) R.Br. by Spiral Countercurrent Chromatography

Year: 2014

Type: article

Abstract: The introduction to high-speed countercurrent chromatography of a spiral tubing support rotor has enabled the application of more polar volatile solvent systems for natural products separation, especially water soluble compounds and their metabolites. Here is reported the use of spiral countercurrent chromatography with the spiral tubing support rotor to fractionate extracts of the African medicinal plant Sutherlandia frutescens (L.) R.Br. A two-phase solvent system of ethyl acetate, methanol, water, and n-butanol, with the lower aqueous phase mobile, separated sutherlandioside B efficiently from other compounds. The purity of sutherlandioside B was determined by high-performance liquid chromatography and mass spectrometry analysis. Sufficient quantities of sutherlandioside B can be purified by this method for use in cell culture assays and the development of a sensitive antibody by which to detect the consumption and metabolism in vivo of this novel botanical.

Source: Journal of Liquid Chromatography & Related Technologies

DOI: 10.1080/10826076.2014.913518

Authors: Korey J. Brownstein, Martha Knight, Yoichiro Ito, George E. Rottinghaus, William R. Folk

Institutions: University of Missouri, National Institutes of Health, National Heart, Lung, and Blood Institute

Language: English

Topic: Chromatography in Natural Products

Subfield: Analytical Chemistry

Field: Chemistry

Domain: Physical Sciences

Open Access status: green

Funders: National Institutes of Health, National Heart, Lung, and Blood Institute, National Center for Complementary and Integrative Health

Awards: TICIPS: HIV/AIDS, Secondary Infections and Immune Modul*, G375128302, Spiral tube assembly for countercurrent chromatography: tubing modification by a simple tool to improve the partition efficincy