Work

Isolation of Caffeic Acid From Eupatorium Adenophorum Spreng by High-speed Countercurrent Chromatography and Synthesis of Caffeic Acid-intercalated Layered Double Hydroxide

Year: 2010

Type: article

Abstract: Preparative high-speed countercurrent chromatography (HSCCC) was successfully used for isolation and purification of caffeic acid from Eupatorium adenophorum Spreng with a solvent system composed of ethyl acetate-methanol-water at a volume ratio of 10:1:10, v/v. Using a preparative unit of the HSCCC centrifuge, about a 938 mg amount of the crude extract was separated, yielding 63.2 mg of caffeic acid at purity of 96.0%. Then, the anti-microbial and anti-virus drug caffeic acid (C(9)H(8)O(4)) was intercalated into layered double hydroxides for the first time by anion exchange under a nitrogen atmosphere. The product caffeic acid-LDH has been characterized by powder X-ray diffraction (XRD), Fourier transform-infrared (FT-IR), Scanning electron micrographs (SEM), indicating that the drug has been successfully intercalated into LDH.

Source: Journal of Liquid Chromatography & Related Technologies

DOI: 10.1080/10826071003684471

Authors: Yun Wei, Yali Gao, Kai Zhang, Yoichiro Ito

Institutions: State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, National Institutes of Health, National Heart, Lung, and Blood Institute

Language: English

Topic: Chromatography in Natural Products

Subfield: Analytical Chemistry

Field: Chemistry

Domain: Physical Sciences

Open Access status: green

Funder: National Institutes of Health

Award: G4705346009