Work

Filter replicas and permanent collections of recombinant DNA plasmids

Year: 1979

Type: article

Abstract: A permanent, ordered collection of 23,000 recombinant DNA plasmids containing Drosophila melanogaster DNA has been established. Simple and practical methods for storing and manipulating this collection were developed. In addition, an improved, simple and inexpensive method for making paper filter replicas of such an ordered collection and of a high density (10,000 colonies/petri dish) unordered collection was developed. These filter replicas are suitable for nucleic acid hybridization screens of recombinant DNA colinies and each filter replica can be used for many (greater than 5) successive screens. The kinetics of this hybridization reaction were examined and allow design of experiments that detect colony complementarity to a nucleic acid that is 0.5% of the hybridization probe.

Source: Nucleic Acids Research

DOI: 10.1093/nar/7.8.2115

Authors: J.Peter Gergen, Ralph H. Stern, Pieter C. Wensink

Institution: Brandeis University

Language: English

Topic: Molecular Biology Techniques and Applications

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

OpenAlex ID: W2059536036

FWCI: 7.425

Cites: 37

Cited by: 348

Related to: 10