Work

Site-directed mutagenesis reveals a liver transcription factor essential for the albumin transcriptional enhancer.

Year: 1990

Type: article

Abstract: A method based on the polymerase chain reaction is described for constructing a clustered set of basepair changes, deletions, or insertions at any site on a DNA fragment. Advantages of the procedure are that virtually every product has the desired sequence alteration and that only a single round of polymerase chain reaction is required. We used this method to demonstrate that the binding of a specific liver nuclear protein, which we call eH-TF, is essential for the function of the enhancer of the mouse albumin gene. The eH-TF binding activity is hepatocyte-specific; it binds to a functional region of the albumin promoter and is distinct from other albumin promoter factors, and part of the eH-TF binding sequence, TGTTTGC, occurs in functional regulatory sites of other liver-specific genes.

Source: Proceedings of the National Academy of Sciences

DOI: 10.1073/pnas.87.14.5469

Authors: K S Zaret, J K Liu, C M DiPersio

Institution: Brown University

Language: English

Topic: Genomics and Chromatin Dynamics

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: McKnight Foundation, Searle Scholars Program, National Institutes of Health, National Institute of General Medical Sciences

Awards: G3123714125, G9000055649