Work

Single-Stranded Deoxyribonucleic Acid-specific Nuclease from Vaccinia Virus

Year: 1974

Type: article

Abstract: A deoxyribonuclease was extracted from vaccinia virus cores and purified 200-fold by chromatography on DEAE-cellulose, DNA-cellulose, and hydroxyapatite. Approximately 0.35 mg of enzyme was isolated from 63 mg of cores. Preparations of [3H]leucine-labeled deoxyribonuclease appeared nearly homogeneous as judged by gel filtration and had a molecular weight of 50,000 as determined by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate. The molecular weight of the native enzyme determined by sucrose gradient sedimentation and filtration through Sephadex G-200 was 105,000, suggesting that it may exist as a dimer. The enzyme hydrolyzed single-stranded DNA most actively at pH 4.4 and did not require divalent cations.

Source: Journal of Biological Chemistry

DOI: 10.1016/s0021-9258(19)42670-7

Authors: Hortencia Rosemond-Hornbeak, Enzo Paoletti, Bernard Moss

Institutions: National Institutes of Health, National Institute of Allergy and Infectious Diseases

Language: English

Topic: Poxvirus research and outbreaks

Subfield: Virology

Field: Immunology and Microbiology

Domain: Life Sciences

Open Access status: hybrid

Estimated APC paid: $3,430

OpenAlex ID: W206034678

FWCI: 10.69

Cites: 36

Cited by: 50

Related to: 10