Work

L factor that is required for beta-galactosidase synthesis is the nusA gene product involved in transcription termination.

Year: 1980

Type: article

Abstract: The DNA-dependent in vitro synthesis of Escherichia coli beta-galactosidase requires the presence of a soluble protein referred to as L factor [Kung, H., Spears, C. & Weissbach, H. (1975) J. Biol. Chem. 250, 1556-1562]. In the present study, comparison of physical, immunological, and biological properties shows that L factor is the product of the E. coli nusA gene. The nusA gene product is known to interact with bacteriophage lambda N gene protein and to prevent premature termination of transcription from the early lambda promoters. Our results suggest that premature transcription termination in the lac operon of E. coli may also be overcome by the nusA protein.

Source: Proceedings of the National Academy of Sciences

DOI: 10.1073/pnas.77.4.1991

Authors: J Greenblatt, J Li, S Adhya, D I Friedman, L S Baron, B Redfield, H F Kung, H Weissbach

Institution: University of Toronto

Language: English

Topic: Bacterial Genetics and Biotechnology

Subfield: Genetics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

OpenAlex ID: W2060452442

FWCI: 9.211

Cites: 21

Cited by: 70

Related to: 10