Work

Norepinephrine‐induced synthesis of the uncoupling protein thermogenin (UCP) and its mitochondrial targeting in brown adipocytes differentiated in culture

Year: 1990

Type: article

Abstract: Synthesis of the brown adipocyte-specific mitochondrial uncoupling protein thermogenin (UCP) is demonstrated here in brown adipocytes differentiated in culture from precursor cells. By immunoblotting, no UCP was detectable in untreated multilocular adipocytes. The synthesis of UCP was stimulated by norepinephrine at physiological concentrations and was observable already after 2 h. It was evident from immunoelectron microscopy that the newly synthesised protein was targeted to the mitochondrial inner membrane, demonstrating the functional competence of these cultured cells.

Source: FEBS Letters

DOI: 10.1016/0014-5793(90)81031-i

Authors: David Herron, Stefan Rehnmark, Myriam Néchad, Dragutin Lončar, Barbara Cannon, Jan Nedergaard

Institutions: Stockholm University, Centre National de la Recherche Scientifique, Université Pierre-et-Marie-Curie

Language: English

Topic: Adipose Tissue and Metabolism

Subfield: Physiology (medical)

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: bronze

Funder: Centre National de la Recherche Scientifique