Work

High-Frequency Flp Recombinase-Mediated Inversions of the oriC -Containing Region of the Pseudomonas aeruginosa Genome

Year: 2000

Type: article

Abstract: The genomes of the two clonally derived Pseudomonas aeruginosa prototypic strains PAO1 and DSM-1707 differ by the presence of a 2. 19-Mb inversion including oriC. Integration of two Flp recombinase target sites near the rrn operons containing the inversion endpoints in PAO1 led to Flp-catalyzed inversion of the intervening 1.59-Mb fragment, including oriC, at high frequencies (83%), favoring the chromosome configuration found in DSM-1707. The results indicate that the oriC-containing region of the P. aeruginosa chromosome can readily undergo and tolerate large inversions.

Source: Journal of Bacteriology

DOI: 10.1128/jb.182.24.7070-7074.2000

Authors: Nazir Barekzi, Kerry Beinlich, Tung T. Hoang, Xuan-Quynh Pham, RoxAnn Karkhoff-Schweizer, Herbert P. Schweizer

Institutions: Colorado State University, University of Washington

Language: English

Topic: Bacterial Genetics and Biotechnology

Subfield: Genetics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: National Institutes of Health, National Institute of General Medical Sciences

Awards: G6275005276, PSEUDOMONAS AUTOINDUCER BIOSYNTHESIS AND DRUG DISCOVERY