Work

Partial characterization of low molecular weight proteoglycan isolated from bovine parietal pericardium

Year: 1988

Type: article

Abstract: Knowledge of the nature of pericardial connective tissue components is incomplete. To gain a better understanding of the composition of this tissue, bovine parietal pericardium was extracted with 4 M guanidine hydrochloride yielding a proteoglycan-containing protein mixture. This was fractionated by a three-step chromatographic procedure with the resultant purification of a 75-110 Kd proteoglycan. The purified proteoglycan was susceptible to chondroitinase ABC digestion but resistant to chondroitinase AC and nitrous acid degradation suggesting the presence of dermatan sulfate glycosaminoglycan(s). This is the first reported isolation of a proteoglycan from parietal pericardium.

Source: Biochemical and Biophysical Research Communications

DOI: 10.1016/0006-291x(88)90618-3

Authors: Dan Simionescu, Robert Alper, Nicholas A. Kefalides

Institutions: University City Science Center, University of Pennsylvania

Language: English

Topic: Proteoglycans and glycosaminoglycans research

Subfield: Cell Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: hybrid

Estimated APC paid: $3,110

Funders: National Heart, Lung, and Blood Institute, National Institute of Arthritis and Musculoskeletal and Skin Diseases