Work

Superresolution by image scanning microscopy using pixel reassignment

Year: 2013

Type: article

Abstract: The effect of detector array size on resolution and signal collection efficiency of image scanning microscopy based on pixel reassignment is studied. It is shown how the method can also be employed if there is a Stokes shift in fluorescence emission wavelength. With no Stokes shift, the width of the point spread function can be sharpened by a factor of 1.53, and its peak intensity increased by a factor of 1.84.

Source: Optics Letters

DOI: 10.1364/ol.38.002889

Authors: Colin J. R. Sheppard, Shalin B. Mehta, Rainer Heintzmann

Institutions: Italian Institute of Technology, Marine Biological Laboratory, Leibniz Institute of Photonic Technology

Language: English

Topic: Advanced Fluorescence Microscopy Techniques

Subfield: Biophysics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: bronze

OpenAlex ID: W2061182600

FWCI: 5.069

Cites: 15

Cited by: 238

Related to: 10