An improved agar-plate method for studying root growth and response of Arabidopsis thaliana
Year: 2013
Type: article
Abstract: Arabidopsis thaliana is a widely used model plant for plant biology research. Under traditional agar-plate culture system (TPG, traditional plant-growing), both plant shoots and roots are exposed to illumination, and roots are grown in sucrose-added medium. This is not a natural environment for the roots and may cause artifact responses. We have developed an improved agar-plate culture system (IPG, improved plant-growing) where shoots are illuminated but roots are grown in darkness without sucrose addition. Compared to TPG, IPG produced plants with significantly less total root length, lateral root length and root hair density, although their primary roots were longer. Root gravitropism, PIN2 (an auxin efflux carrier) abundance, H⁺ efflux or Ca²⁺ influx in root apexes, were weaker in IPG-grown roots than those in TPG-grown roots. We conclude that IPG offers a more natural way to study the root growth and response of Arabidopsis thaliana.
Source: Scientific Reports
DOI: 10.1038/srep01273
Institutions: Chinese University of Hong Kong, Fujian Agriculture and Forestry University, University of Bonn, Shandong Agricultural University, Chinese Academy of Sciences, Institute of Soil Science, State Key Laboratory of Soil and Sustainable Agriculture, Southern University of Science and Technology
Language: English
Subfield: Plant Science
Domain: Life Sciences
SDG: Zero hunger
Open Access status: gold
Estimated APC paid: $2,690
OpenAlex ID: W2061236377