Work

Detecting DNA Depurination with Solid-State Nanopores

Year: 2014

Type: article

Abstract: Among the different types of DNA damage that occur endogenously in the cell, depurination is especially prevalent. These lesions can initiate mutagenesis and have been implicated in a variety of diseases, including cancer. Here, we demonstrate a new approach for the detection of depurination at the single-molecule scale using solid-state nanopores. We induce depurination in short duplex DNA using acidic conditions and observe that the presence of apurinic sites results in significantly slower dynamics during electrokinetic translocation. This procedure may be valuable as a diagnostic for in situ quantification of DNA depurination.

Source: PLoS ONE

DOI: 10.1371/journal.pone.0101632

Authors: Michael M. Marshall, Jan A. Ruzicka, Ethan W. Taylor, Adam R. Hall

Institutions: University of North Carolina at Greensboro, Wake Forest University Health Sciences, Wake Forest University

Language: English

Topic: Nanopore and Nanochannel Transport Studies

Subfield: Biomedical Engineering

Field: Engineering

Domain: Physical Sciences

Open Access status: gold

Estimated APC paid: $2,382

Funder: Wake Forest University