Year: 2010
Type: article
Abstract: Optical absorption provides essential biological functional information but cannot be sensed by mainstream optical microscopy technologies directly, which detect fluorescence or scattering and may require undesirable labeling. Here we developed in vivo subwavelength-resolution photoacoustic microscopy (SW-PAM) that provides exquisitely high optical-absorption contrast due to nonfluorescent, or fluorescent, endogenous pigments. Having approached the ultimate diffraction-limited optical resolution, SW-PAM can resolve subcellular organelles. Vasculature and early-stage melanoma were imaged with 12:1 and 17:1 contrasts, respectively, without labeling. SW-PAM along with the scaled-up macroscopy, as the only technology that measures the same contrast origin over such a wide length scale, can potentially accelerate translation from microscopic research to clinical practice.
Source: Optics Letters
DOI: 10.1364/ol.35.003195
Authors: Chi Zhang, Konstantin Maslov, Lihong V. Wang
Institution: Washington University in St. Louis
Language: English
Subfield: Biomedical Engineering
Field: Engineering
Domain: Physical Sciences
Open Access status: bronze
Awards: Diabetes Research & Training Center, RECOVERY OF OPTICAL ABSORPTION COEFFICIENT IN QUANTITATIVE PHOTOACOUSTIC IMAGING, G3341232771, Confocal Photacoustic Microscopy: Technology Development, G4445481002, G6096271865, Photoacoustic / Optical / Ultrasonic Imaging of Sentinel Lymph Nodes and Metastas, Monitoring of Breast Neoadjuvant Therapy by Thermo- and Photo- Acoustic Tomograph
OpenAlex ID: W2061707289