Work

Intracellular Cleavage of Notch Leads to a Heterodimeric Receptor on the Plasma Membrane

Year: 1997

Type: article

Abstract: Previous models for signal transduction via the Notch pathway have depicted the full-length Notch receptor expressed at the cell surface. We present evidence demonstrating that the Notch receptor on the plasma membrane is cleaved. This cleavage is an evolutionarily conserved, general property of Notch and occurs in the trans-Golgi network as the receptor traffics toward the plasma membrane. Although full-length Notch is detectable in the cell, it does not reach the surface. Cleavage results in a C-terminal fragment, N(TM), that appears to be cleaved N-terminal to the transmembrane domain, and an N-terminal fragment, N(EC), that contains most of the extracellular region. We provide evidence that these fragments are tethered together on the plasma membrane by a link that is sensitive to reducing conditions, forming a heterodimeric receptor.

Source: Cell

DOI: 10.1016/s0092-8674(00)80336-0

Authors: Christine M Blaumueller, Huilin Qi, Panayiotis Zagouras, Spyros Artavanis-Tsakonas

Institutions: Howard Hughes Medical Institute, Yale University

Language: English

Topic: Developmental Biology and Gene Regulation

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: hybrid

Estimated APC paid: $11,400

Funder: National Institute of Neurological Disorders and Stroke