Lack of Molecular Correlates of Plasmodium vivax Ookinete Development
Year: 2011
Type: article
Abstract: Previous studies of Plasmodium vivax transmission to Anopheles spp. mosquitoes have not been able to predict mosquito infectivity on the basis of microscopic or molecular quantification of parasites (total parasites in the sample or total number of gametocytes) in infected blood. Two methods for production of P. vivax ookinete cultures in vitro, with yields of 10(6) macrogametocytes, 10(4) zygotes, and 10(3) ookinetes, respectively, per 10 mL of P. vivax-infected patient blood with approximately 0.01% parasitemia, were used to study P. vivax sexual stage development. The quantity of gametocytes, determined by counting Giemsa-stained blood smears, and quantity and type of gametocyte as determined by quantitative reverse transcriptase-polymerase chain reaction for Pvalpha tubulin II and macrogametocyte-specific pvg377 did not predict ookinete yield. Factors that affect the efficiency of in vitro P. vivax ookinete transformation remain poorly understood.
Language: English
Topic: Malaria Research and Control
Field: Medicine
Domain: Health Sciences
Open Access status: green
Awards: Molecular Targets of Blocking Malaria Transmission, G3188613685, Amazonian Center of Excellence in Malaria Research, Patient-Oriented Research in Tropical Infectious Diseases, Translational Research Development for Endemic Infectious Diseases of Amazonia, Medical Scientist Training Program, Human Reservoirs of Plasmodium vivax Transmission in the Peruvian Amazon
OpenAlex ID: W2062424197