Year: 2015
Type: article
Abstract: Animal models of epilepsy are critical not only for understanding the fundamental mechanism of epilepsy but also for testing the efficacy of new antiepileptic drugs and novel therapeutic interventions. Photorelease of caged molecules is widely used in biological research to control pharmacologic events with high spatio-temporal resolution. We developed a technique for in vivo optical triggering of neocortical seizures using a novel caged compound based on ruthenium photochemistry (RuBi-4AP). Epileptiform events in mouse cortex were induced with blue light in both whole brain and focal illumination. Multi-electrode array recording and optical techniques were used to characterize the propagation of these epileptic events, including interictal spikes, polyspikes, and ictal discharges. These results demonstrate a novel optically-triggered seizure model, with high spatio-temporal control, that could have widespread application in the investigation of ictal onset, propagation and to develop novel light-based therapeutic interventions.
Source: Frontiers in Neuroscience
Language: English
Subfield: Psychiatry and Mental health
Field: Medicine
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $3,801
Funders: W. M. Keck Foundation, Wellcome Trust, Universidad de Buenos Aires, Defense Advanced Research Projects Agency, Multidisciplinary University Research Initiative, National Institute of Mental Health, National Institute on Drug Abuse, National Eye Institute, National Institute of Neurological Disorders and Stroke, National Center for Research Resources, Army Research Office, Army Research Laboratory
Awards: G1242600127, G2723399458, G3205720133, Understanding epilepsy in the active brain., G4012432327, Optical imaging of epilepsy in rat and human neocortex, G5259331294, G6323555837, Imaging Functional Connectivity in Visual Cortex, Functional connectomics of the neocortical microcircuit, G7452299184, Astrocytic regulation of neuronal synchronization, G8340509167, Two-photon optical control of astrocytic function, Novel caged Dopamine compounds, Clinical and Translational Science Center (UL1)
OpenAlex ID: W2062887429