Work

Fluorometric Detection of Inositol Phosphates and the Activity of Their Enzymes with Synthetic Pores: Discrimination of IP7 and IP6 and Phytate Sensing in Complex Matrices

Year: 2008

Type: article

Abstract: We report the fluorometric and noninvasive detection of inositol phosphates, which act as privileged blockers of synthetic pores. Phytate (IP 6 ) and IP 7 recognition in the pore occurs substoichiometrically in the low nanomolar range, with more than 2 orders of magnitude higher sensitivity than the best available alternative. Zn 2+ -mediated fluorometric discrimination between IP 6 and IP 7 demonstrates that significant pore discrimination challenges can be solved with judiciously selected additives. The detectability of inositol phosphate enzyme activity was exemplified with phytase. Phytate sensing was accomplished in complex matrices such as extracts from almonds, soybeans, or lentils, using phytase as a specific signal generator. These results are important because they not only add essential evidence in support of the usefulness of synthetic pores as multianalyte sensors in complex matrices but also reveal the existence of privileged analytes that can provide access to submicromolar sensitivity without the need of refined sensing strategies.

Source: Journal of the American Chemical Society

DOI: 10.1021/ja710186e

Authors: Sara M. Butterfield, Duy-Hien Tran, Honglu Zhang, Glenn D. Prestwich, Stefan Matile

Institutions: University of Geneva, University of Utah

Language: English

Topic: Molecular Sensors and Ion Detection

Subfield: Spectroscopy

Field: Chemistry

Domain: Physical Sciences

Open Access status: green

Funders: National Institutes of Health, National Institute of Neurological Disorders and Stroke

Awards: G6895356147, Phosphoinositide and Inositol Polyphosphate Probes