Immobilization of DNA via oligonucleotides containing an aldehyde or carboxylic acid group at the 5' terminus
Year: 1987
Type: article
Abstract: A general method for the immobilization of DNA through its 5'-end has been developed. A synthetic oligonucleotide, modified at its 5'-end with an aldehyde or carboxylic acid, was attached to latex microspheres containing hydrazide residues. Using T4 polynucleotide ligase and an oligonucleotide splint, a single stranded 98mer was efficiently joined to the immobilized synthetic fragment. After impregnation of the latex microspheres with the fluorescent dye, Nile Red and attachment of an aldehyde 16mer, 5 X 10(5) bead-DNA conjugates could be detected with a conventional fluorimeter.
Source: Nucleic Acids Research
Authors: Jonathan N. Kremsky, Joseph L. Wooters, Joseph P. Dougherty, Rachel E. Meyers, Mary Collins, Eugene L. Brown
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W2063601748