Work

Functional Characterization of ttnI Completing the Tailoring Steps for Tautomycetin Biosynthesis in Streptomyces griseochromogenes

Year: 2012

Type: article

Abstract: The tautomycetin (TTN) biosynthetic gene cluster has been recently cloned and sequenced from Streptomyces griseochromogenes, unveiling four genes, ttnCDFI, as candidates to encode the tailoring steps for TTN biosynthesis. It is reported that (i) TtnC plays no essential role in TTN biosynthesis, (ii) TtnI catalyzes C-5 oxidation, and (iii) combining the previous findings with TtnFD, the tailoring steps from TTN F-1 to TTN take place in the order of TtnF-catalyzed C-1"/C-2" dehydration, TtnD-catalyzed C-3" decarboxylation, and TtnI-catalyzed C-5 oxidation.

Source: Organic Letters

DOI: 10.1021/ol300187p

Authors: Dong Yang, Wenli Li, Sheng-Xiong Huang, Ben Shen

Institutions: Scripps Research Institute, University of Wisconsin–Madison

Language: English

Topic: Microbial Natural Products and Biosynthesis

Subfield: Pharmacology (medical)

Field: Medicine

Domain: Health Sciences

Open Access status: green

Funder: National Cancer Institute

Awards: UWCCC National Cooperative Drug Discovery Group for Can*, G2271657281