Work

Strand exchange in site-specific recombination.

Year: 1979

Type: article

Abstract: The site-specific recombination system of phage lambda promotes crossovers at its attachment site (att). In this report we show that when phage are crossed in conditions where only the site-specific recombination system is active, a low frequency of crossovers can also be detected in a region that is close to but does not contain att. These crossovers require the phage int gene, the host hip gene, and the integrity of att. They are not detected if one of the parents carries a substitution of a heterologous attachment site (attB instead of attP). To explain these findings we suggest that site-specific recombination can proceed by exchange of single strands between the participating chromosomes at att and migration of the resulting junction outside of att.

Source: Proceedings of the National Academy of Sciences

DOI: 10.1073/pnas.76.3.1363

Authors: L W Enquist, H Nash, R A Weisberg

Institutions: Princeton University, National Institutes of Health

Language: English

Topic: Bacterial Genetics and Biotechnology

Subfield: Genetics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

OpenAlex ID: W2063954098

FWCI: 2.123

Cites: 27

Cited by: 29

Related to: 10