Work

Sizing up metatranscriptomics

Year: 2012

Type: review

Abstract: A typical marine bacterial cell in coastal seawater contains only ∼200 molecules of mRNA, each of which lasts only a few minutes before being degraded. Such a surprisingly small and dynamic cellular mRNA reservoir has important implications for understanding the bacterium's responses to environmental signals, as well as for our ability to measure those responses. In this perspective, we review the available data on transcript dynamics in environmental bacteria, and then consider the consequences of a small and transient mRNA inventory for functional metagenomic studies of microbial communities.

Source: The ISME Journal

DOI: 10.1038/ismej.2012.94

Authors: Mary Ann Moran, Brandon Satinsky, Scott M Gifford, Haiwei Luo, Adam Rivers, Leong-Keat Chan, Jun Meng, Bryndan P Durham, Chen Shen, Vanessa A Varaljay, Christa B Smith, Patricia L Yager, Brian M Hopkinson

Institutions: University of Georgia, Massachusetts Institute of Technology, Joint Genome Institute

Language: English

Topic: Genomics and Phylogenetic Studies

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Life below water

Open Access status: bronze

Funders: National Science Foundation, Gordon and Betty Moore Foundation

Awards: MO: Functional Genomic Investigations of Dissolved Organic Carbon Cyling at the Sapelo Island Microbial Observatory, Collaborative Research: Physiological and Genetic Characterization of C02 Concentrating Mechanisms in Marine Diatoms

OpenAlex ID: W2064334669

FWCI: 1.721

Cites: 50

Cited by: 368

Related to: 9