Work

Cross‐linking of the B cell receptor induces activation of phospholipase D through Syk, Btk and phospholipase C‐γ2

Year: 1999

Type: article

Abstract: Phospholipase D (PLD) has been proposed to play a key role in the signal transduction of cellular responses to various extracellular signals. Herein we provide biochemical and genetic evidence that cross-linking of the B cell receptor (BCR) induces rapid activation of PLD through a Syk-, Btk- and phospholipase C (PLC)-gamma2-dependent pathway in DT40 cells. Activation of PLD upon BCR engagement is completely blocked in Syk- or Btk-deficient cells, but unaffected in Lyn-deficient cells. Furthermore, in PLC-gamma2-deficient cells, BCR engagement failed to activate PLD. These results demonstrate that Syk, Btk and PLC-gamma2 are essential for BCR-induced PLD activation.

Source: FEBS Letters

DOI: 10.1016/s0014-5793(99)00153-2

Authors: Tomohiro Hitomi, Shigeru Yanagi, Ryoko Inatome, Hirohei Yamamura

Institution: Kobe University

Language: English

Topic: Protein Kinase Regulation and GTPase Signaling

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: bronze

OpenAlex ID: W2064352346

FWCI: 0.766

Cites: 38

Cited by: 28

Related to: 10