Evaluation of OSOM Trichomonas rapid test for diagnosis of Trichomonas vaginalis infection in females
Year: 2010
Type: article
Study design: Observational Study
Abstract: Background: Trichonomas vaginalis is a parasitic protozoan, sexually transmitted world wide. WHO estimates show that this infection accounts for almost half of all curable sexually transmitted infections. Diagnosis is usually done by observing motile parasite from vaginal or cervical swab specimens by direct wet mount examination. Other diagnostic test includes geimsa staining, fluorescent staining and culture.. OSOM rapid test (Genzyme Diagnostics Cambridge Massachusettes) based on immunochromatographic capillary flow dipstick is newer method available for diagnosis of sexually transmitted disease. Objectives: To compare wet mount examination, giemsa staining and acridine orange staining, culture as diagnostic test for Trichomonas Vaginalis To compare sensitivity, specificity of OSOM trichomonas rapid test with culture and different staining techniques vailable for diagnosis of this sexually transmitted disease. Methods: The study was carried out on 405 aged 15-45 years patients attending gynecological out patient department with complaint of vaginal discharge. Four high vaginal swabs from posterior fornix of vagina were taken from each patient. One swab used for preparation of wet mount, one for making smearsof giemsa and acridine orange staining and other for OSOM trichomonas rapid test and culture done on kupferberg culture medium. Results: Out of 405 patient of vaginal discharge included in the study, Wet mount and giemsa staining showed T.vaginalis in 7 (1.7%) cases each. Acridine orange staining was positive in 12 cases (3.0%), while OSOM rapid test detected vaginalis infection in 15 cases. Culture on kupferberg medium was positive in 18 (4.4%) patient, sensitivity; specificity of wet mount examination was found to be 38.9%, 100%, positive and negative predictive values, 100% and 97.2%. Giemsa and acridine orange staining showed sensitivity of 38.9% and 66.67% and specificity 100% each. OSOM rapid test was found to be most sensitive when compared to wet mount examination and staini8ng techniques taking culture on kupferberg medium as gold standard, while staining methods and OSOM test were found to be equally specific. Conclusion: OSOM rapid test can be used as adjunct to microscopy and culture. Its high sensitivity and specificity advocates its use in settings were culture is not possible as direct microscopic examination and different staining techniques fails to detect infection in large number of cases. Abstracts for SupplementInternational Journal of Infectious DiseasesVol. 14Preview Full-Text PDF Open Archive
Authors: F. Shujatullah, H.M. Khan, R. Khatoon, T. Rabbani, M. Shahid, A. Raza
Language: English
Subfield: Microbiology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $2,900
OpenAlex ID: W2064404303