The NtAMI1 gene functions in cell division of tobacco BY‐2 cells in the presence of indole‐3‐acetamide
Year: 2008
Type: article
Abstract: Tobacco (Nicotiana tabacum) Bright Yellow-2 (BY-2) cells can be grown in medium containing indole-3-acetamide (IAM). Based on this finding, the NtAMI1 gene, whose product is functionally equivalent to the AtAMI1 gene of Arabidopsis thaliana and the aux2 gene of Agrobacterium rhizogenes, was isolated from BY-2 cells. Overexpression of the NtAMI1 gene allowed BY-2 cells to proliferate at lower concentrations of IAM, whereas suppression of the NtAMI1 gene by RNA interference (RNAi) caused severe growth inhibition in the medium containing IAM. These results suggest that IAM is incorporated into plant cells and converted to the auxin, indole-3-acetic acid, by NtAMI1.
Source: FEBS Letters
Authors: Keiichirou Nemoto, Masamitsu Hara, Masashi Suzuki, Hikaru Seki, Toshiya Muranaka, Yoshihiro Mano
Institutions: Tokai University, RIKEN, Yokohama City University
Language: English
Subfield: Plant Science
Domain: Life Sciences
SDG: Zero hunger
Open Access status: bronze
OpenAlex ID: W2064770128