Revealing the role of phospholipase Cβ3 in the regulation of VEGF-induced vascular permeability
Year: 2012
Type: article
Abstract: VEGF induces vascular permeability (VP) in ischemic diseases and cancer, leading to many pathophysiological consequences. The molecular mechanisms by which VEGF acts to induce hyperpermeability are poorly understood and in vivo models that easily facilitate real-time, genetic studies of VP do not exist. In the present study, we report a heat-inducible VEGF transgenic zebrafish (Danio rerio) model through which VP can be monitored in real time. Using this approach with morpholino-mediated gene knock-down and knockout mice, we describe a novel role of phospholipase Cβ3 as a negative regulator of VEGF-mediated VP by regulating intracellular Ca2+ release. Our results suggest an important effect of PLCβ3 on VP and provide a new model with which to identify genetic regulators of VP crucial to several disease processes.
Source: Blood
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
Awards: G1824339530, G1877201681, G2706384329, Distinct Pathways of VPF/VEGF Receptors, Tumor Microenvironment/Angiogenesis Training Grant, G4484623862, Targeting Pancreatic Cancer Using Peptide Chemistry: From Bench to Bedside, REGULATORY PATHWAYS AND ROLE OF VPF/VEGF IN RENAL CANCER, Regulatory Pathways and Role of VPF/VEGF in Renal Cancer, Mayo Center for Cell Signaling in Gastroenterology
OpenAlex ID: W2064889370