Work

Intrinsic Rates and Activation Free Energies from Single-Molecule Pulling Experiments

Year: 2006

Type: article

Abstract: We present a unified framework for extracting kinetic information from single-molecule pulling experiments at constant force or constant pulling speed. Our procedure provides estimates of not only (i) the intrinsic rate coefficient and (ii) the location of the transition state but also (iii) the free energy of activation. By analyzing simulated data, we show that the resulting rates of force-induced rupture are significantly more reliable than those obtained by the widely used approach based on Bell's formula. We consider the uniqueness of the extracted kinetic information and suggest guidelines to avoid over-interpretation of experiments.

Source: Physical Review Letters

DOI: 10.1103/physrevlett.96.108101

Authors: Olga Dudko, Gerhard Hummer, Attila Szabo

Institutions: National Institutes of Health, Center for Information Technology, National Institute of Diabetes and Digestive and Kidney Diseases

Language: English

Topic: Force Microscopy Techniques and Applications

Subfield: Atomic and Molecular Physics, and Optics

Field: Physics and Astronomy

Domain: Physical Sciences

Open Access status: green

Funder: National Institutes of Health

OpenAlex ID: W2065907078

FWCI: 26.16

Cites: 22

Cited by: 834

Related to: 10