Year: 2014
Type: article
Abstract: Background Mapping the binding regions of mRNA-binding proteins is critical to the understanding of their regulatory roles in cellular processes. Recent development in experimental technologies combines high throughput sequencing with crosslink immunoprecipitation (HITS-CLIP), which has the merit of detecting RNA-protein interaction sites at a high resolution to single nucleotide level. Analysis of such data typically involves many steps, and teasing out true signals from noise requires crosslink induced mutations (CIMS) analysis, peak identification, integration of the two signal types, and some downstream analysis such as motif finding and conservation evaluation. To our knowledge, there is a lack of a single computational tool that can perform all the tasks as mentioned in an easily accessible manner. Despite the fact that there are several tools available, each performs an individual task.
Source: BMC Bioinformatics
Authors: Xue Zhong, Qi Liu, Yu Shyr
Institutions: Vanderbilt University, Vanderbilt University Medical Center
Language: English
Topic: RNA Research and Splicing
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $2,990
OpenAlex ID: W2065993295