Work

High-Throughput Nonlinear Optical Microscopy

Year: 2013

Type: review

Abstract: High-resolution microscopy methods based on different nonlinear optical (NLO) contrast mechanisms are finding numerous applications in biology and medicine. While the basic implementations of these microscopy methods are relatively mature, an important direction of continuing technological innovation lies in improving the throughput of these systems. Throughput improvement is expected to be important for studying fast kinetic processes, for enabling clinical diagnosis and treatment, and for extending the field of image informatics. This review will provide an overview of the fundamental limitations on NLO microscopy throughput. We will further cover several important classes of high-throughput NLO microscope designs with discussions on their strengths and weaknesses and their key biomedical applications. Finally, this review will close with a perspective of potential future technological improvements in this field.

Source: Biophysical Journal

DOI: 10.1016/j.bpj.2013.08.051

Authors: Peter T.C. So, Elijah Y.S. Yew, Christopher Rowlands

Institutions: Singapore-MIT Alliance for Research and Technology, Massachusetts Institute of Technology

Language: English

Topic: Advanced Fluorescence Microscopy Techniques

Subfield: Biophysics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: hybrid

Estimated APC paid: $3,000

Funders: Wellcome Trust, U.S. Public Health Service, National Institute of Neurological Disorders and Stroke, National Institute of Biomedical Imaging and Bioengineering