Work

The human carcinoma cell line A431 possesses large numbers of functional β‐adrenergic receptors

Year: 1984

Type: article

Abstract: The existence of beta-adrenergic receptors was demonstrated on whole A431 cells as well as A431 membrane preparations by means of binding assays using the hydrophobic 1-[3H]dihydroalprenolol and the hydrophilic antagonist [3H]CGP-12,177 as beta-adrenergic ligands. Binding was stereospecific. The receptors, as shown by competition studies, proved to be of the beta 2-subtype and appeared functional in the stimulation of adenylate cyclase. The number of receptors per cell and the yield of receptor sites/mg membrane protein render the A431 cell a useful tool for the study of human beta-adrenergic receptors.

Source: FEBS Letters

DOI: 10.1016/0014-5793(84)80308-7

Authors: C. Delavier-Klutchko, J. Hoebeke, A.D. Strosberg

Institutions: Centre National de la Recherche Scientifique, Institut Jacques Monod

Language: English

Topic: Receptor Mechanisms and Signaling

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: bronze

Funders: Association pour la Recherche sur le Cancer, Institut National de la Santé et de la Recherche Médicale, Centre National de la Recherche Scientifique, National Cancer Institute