Work

Autopsy of a cell

Year: 2014

Type: article

Abstract: Staining with annexin V, which detects the externalization of phosphatidylserine on cells, is a widespread method for the assessment of cell death, and in particular, apoptosis. 1 Although additional assays are important for demonstrating apoptosis, many studies use annexin V staining in isolation and directly equate annexin V positivity with apoptosis. Is this a problem? Although cells undergoing apoptosis externalize phosphatidylserine, describing annexin V-positive cells as apoptotic can be misleading. Here we briefly highlight strengths and limitations of assessing apoptosis using annexin V staining.

Source: Leukemia

DOI: 10.1038/leu.2014.17

Authors: L J Bendall, D R Green

Institutions: The University of Sydney, Westmead Institute, St. Jude Children's Research Hospital

Language: English

Topic: Cell death mechanisms and regulation

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: hybrid

Estimated APC paid: $5,190