Work

β2‐Adrenergic Receptor Solutions for Structural Biology Analyzed with Microscale NMR Diffusion Measurements

Year: 2012

Type: article

Abstract: Microcoil NMR measurements were performed to determine the final composition of solutions of the β(2)-adrenergic receptor (β(2)AR) reconstituted with a detergent and to study the hydrodynamic properties of the detergent micelles containing β(2)AR. Standards are established for the reproducible preparation of G-protein-coupled receptor solutions for crystallization trials and solution NMR studies.

Source: Angewandte Chemie International Edition

DOI: 10.1002/anie.201205474

Authors: Reto Horst, Pawel Stanczak, Raymond C. Stevens, Kurt Wüthrich

Institution: Scripps Research Institute

Language: English

Topic: Receptor Mechanisms and Signaling

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funder: National Institute of General Medical Sciences

Awards: Joint Center for Structural Genomics, JCIMPT-Complexes, GPCR Network