Year: 2011
Type: article
Abstract: The aim of the present study was to investigate the effect of ZnPcS(2)P(2)-meditated sonodynamic therapy (SDT) on U251 human glioma cells and to identify its underlying biological mechanism. The growth inhibition rate was determined by MTT assay. The apoptotic rate was examined by flow cytometry. Fine structures were observed with transmission electron microscopy (TEM). Generation of reactive oxygen species (ROS) was detected spectrophotometrically. Caspase-3, -8 and -9 expression was detected by Western blot analysis. The growth inhibition rate of U251 human glioma cells indicated that ZnPcS(2)P(2)-meditated SDT had a better growth inhibition rate of tumor cells at a concentration of 5.0 μg/ml ZnPcS(2)P(2), at a 4-h incubation time with ZnPcS(2)P(2), and at 6 h re-incubation following SDT. At 6 h after SDT, the growth inhibition rate of cells was significantly higher compared to other groups, apoptosis could be detected in SDT by flow cytometry. TEM examination revealed morphological features of apoptosis or necrosis. Furthermore, caspase-3, -8 and -9 expression following SDT was found to be increased by Western blot analysis. Finally, generation of ROS in cells was also elevated. In conclusion, ZnPcS(2)P(2)-SDT is capable of inducing U251 cell apoptosis or necrosis and has satisfying antitumor effects. The mechanism of ZnPcS(2)P(2)-meditated SDT involves ROS generation in U251 cells, which initiates subsequent apoptosis through the mitochondrial and death receptor pathways.
DOI: 10.3892/etm.2011.390
Authors: ZHIQIANG CHEN, JIANHUA LI, XUEMING SONG, ZHI WANG, WU YUE
Language: English
Subfield: Pulmonary and Respiratory Medicine
Field: Medicine
Domain: Health Sciences
Open Access status: bronze
Funder: National Science Foundation
OpenAlex ID: W2067031197