Work

DNA‐Coated AFM Cantilevers for the Investigation of Cell Adhesion and the Patterning of Live Cells

Year: 2008

Type: article

Abstract: Get a grip: Measurement of receptor adhesion strength requires the precise manipulation of single cells on a contact surface. To attach live cells to a moveable probe, DNA sequences complementary to strands displayed on the plasma membrane are introduced onto AFM cantilevers (see picture, bp=base pairs). The strength of the resulting linkages can be tuned by varying the length of DNA strands, allowing for controlled transport of the cells.

Source: Angewandte Chemie International Edition

DOI: 10.1002/anie.200802525

Authors: Sonny C. Hsiao, Ailey K. Crow, Wilbur A. Lam, Carolyn R. Bertozzi, Daniel A. Fletcher, Matthew B. Francis

Institutions: Lawrence Berkeley National Laboratory, University of California, Berkeley, University of California, San Francisco, Howard Hughes Medical Institute

Language: English

Topic: Force Microscopy Techniques and Applications

Subfield: Atomic and Molecular Physics, and Optics

Field: Physics and Astronomy

Domain: Physical Sciences

Open Access status: green

Funders: Howard Hughes Medical Institute, National Institute of General Medical Sciences

Award: Differential Force Microscopy