Work

In vitro enzymatic biodegradation of adhesive resin in the hybrid layer

Year: 2010

Type: article

Abstract: Penetration of adhesives into the demineralized dentin surface and their subsequent conversion are critically important to longevity of the adhesive resin (AR)-dentin bond. The durability of the resin-dentin bond is investigated by monitoring the change of adhesive concentration within the hybrid layer (HL) of aged specimens using Raman spectroscopy. Absolute molar concentrations of Bis-GMA and HEMA were measured across the HL of resin-dentin specimens 24 h after photopolymerization and after 24-week storage in one of three media: artificial saliva (SAL), SAL containing cholesterol esterase to attack resin (EST), and SAL containing bacterial collagenase to attack collagen (COL). No significant difference among these groups for both Bis-GMA and HEMA molar concentrations at 24-h storage was found; however, concentrations decreased from the AR to the middle of the HL. Concentrations remained unchanged at any resin-dentin position after aging in SAL. In the HL, concentrations significantly decreased with aging in COL and tended to decrease in EST. While showing potential enzymatic biodegradative effects of endogenous matrix metalloproteinases and salivary esterases, this methodology may also prove to be a valuable assessment of new chemistries and future approaches to improve resin-dentin bond performance. (c) 2010 Wiley Periodicals, Inc. J Biomed Mater Res, 2010.

Source: Journal of Biomedical Materials Research Part A

DOI: 10.1002/jbm.a.32689

Authors: Yuan Zou, Julie L. P. Jessop, Steven R. Armstrong

Institution: University of Iowa

Language: English

Topic: Dental materials and restorations

Subfield: Orthodontics

Field: Dentistry

Domain: Health Sciences

Open Access status: green

Funder: National Institute of Dental and Craniofacial Research

Awards: G1225869114, Biodurability of Dentin-Adhesive Resin Bond Joint