Work

Transgenic Mouse Sperm that Have Green Acrosome and Red Mitochondria Allow Visualization of Sperm and Their Acrosome Reaction in Vivo

Year: 2010

Type: article

Abstract: In the present paper, we introduce a transgenic mouse line whose sperm express green fluorescent protein (GFP) in their acrosome and red fluorescent protein (RFP) in their mitochondria [B6D2F1- Tg(CAG/su9-DsRed2, Acr3-EGFP)RBGS002Osb]. The dual fluorescent sperm showed normal fertilizing ability in both in vivo and in vitro fertilization and the sperm could be observed through uterine and oviductal walls when female reproductive tracts were dissected out and placed under excitation light. This characteristic could facilitate examination of sperm migration inside the female reproductive tract as well as facilitating in situ live imaging of the acrosome reaction, the details of which have remained elusive.

Source: EXPERIMENTAL ANIMALS

DOI: 10.1538/expanim.59.105

Authors: Hidetoshi HASUWA, Yuko MURO, Masahito IKAWA, Noriko KATO, Yoshihide TSUJIMOTO, Masaru OKABE

Institutions: Research Institute for Microbial Diseases, The University of Osaka, The University of Osaka

Language: English

Topic: Sperm and Testicular Function

Subfield: Reproductive Medicine

Field: Medicine

Domain: Health Sciences

Open Access status: bronze

OpenAlex ID: W2067633155

FWCI: 4.563

Cites: 5

Cited by: 171

Related to: 10