Work

Importance of Abscisic Acid (ABA) in the In Vitro Conservation of Cassava (Manihot esculenta Crantz)

Year: 2008

Type: article

Abstract: The conventional technology for in vitro plant conservation for cassava (Manihot esculenta Crantz) germplasm collections is laborious due to the need for several subculturing procedures per year.This practice implies high costs for medium preparation, tissue culture tubes, timeconsuming labor, risks of contamination, mislabeling of accession, and the need for large growth chambers.We have developed a new procedure using in vitro cultivated nodal axillary buds treated with different abscisic acid (ABA) concentrations to reduce the time for recycling transplants cultivated in a SP basic nutritive medium.Nodal explants were stored for three months with ABA.Plants were obtained after nodal axillary buds were placed in SP medium without ABA.Results indicated that 20 and 30 µM ABA induced bud dormancy and delayed sprouting without affecting subsequent growth of plants after treatment.

Source: Chilean journal of agricultural research

DOI: 10.4067/s0718-58392008000300011

Authors: L. Pedro Barrueto Cid, Luiz L.C.B Carvalho

Institutions: Embrapa, Embrapa Recursos Genéticos e Biotecnologia

Language: English

Topic: Plant tissue culture and regeneration

Subfield: Plant Science

Field: Agricultural and Biological Sciences

Domain: Life Sciences

SDG: Zero hunger

Open Access status: diamond

Funders: International Atomic Energy Agency, Embrapa Recursos Genéticos e Biotecnologia