Work

Arabidopsis ABI5 Subfamily Members Have Distinct DNA-Binding and Transcriptional Activities

Year: 2002

Type: article

Abstract: A small family of novel basic leucine zipper proteins that includes abscisic acid (ABA)-INSENSITIVE 5 (ABI5) binds to the promoter region of the lea class gene Dc3. The factors, referred to as AtDPBFs (Arabidopsis Dc3 promoter-binding factors), were isolated from an immature seed cDNA library. AtDPBFs bind to the embryo specification and ABA-responsive elements in the Dc3 promoter and are unique in that they can interact with cis-elements that do not contain the ACGT core sequence required for the binding of most other plant basic leucine zipper proteins. Analysis of full-length cDNAs showed that at least five different Dc3 promoter-binding factors are present in Arabidopsis seeds; one of these, AtDPBF-1, is identical to ABI5. As expected, AtDPBF-1/ABI5 mRNA is inducible by exogenous ABA in seedlings. Despite the near identity in their basic domains, AtDPBFs are distinct in their DNA-binding, dimerization, and transcriptional activity.

Source: PLANT PHYSIOLOGY

DOI: 10.1104/pp.003566

Authors: Soo Young Kim, Jianzhong Ma, Philippe Perret, Zhongsen Li, Terry L. Thomas

Institution: Texas A&M University

Language: English

Topic: Plant Stress Responses and Tolerance

Subfield: Plant Science

Field: Agricultural and Biological Sciences

Domain: Life Sciences

Open Access status: bronze

Funder: U.S. Department of Agriculture

OpenAlex ID: W2068286637