Work

Liquid chromatographic assay of ketoconazole

Year: 1981

Type: article

Abstract: A reverse-phase, high-pressure liquid chromatographic method for the rapid and quantitative determination of ketoconazole has been developed. Drug levels from 0.5 to 10 microgram/ml can be determined in either yeast nitrogen base medium or human serum by using an octadecylsilane column. A retention time of 4.9 +/- 0.1 min resulted when the drug was eluted from a column with 75% methanol-25% 0.02 M (pH 7.5) phosphate buffer at a flow rate of 2 ml/min. Optimum sensitivity was obtained at a wavelength of 231 nm.

Source: Antimicrobial Agents and Chemotherapy

DOI: 10.1128/aac.19.1.110

Authors: F A Andrews, L R Peterson, W H Beggs, D Crankshaw, G A Sarosi

Institutions: United States Department of Veterans Affairs, Veterans Health Administration

Language: English

Topic: Analytical Methods in Pharmaceuticals

Subfield: Analytical Chemistry

Field: Chemistry

Domain: Physical Sciences

Open Access status: green

OpenAlex ID: W2068309382

FWCI: 4.868

Cites: 3

Cited by: 27

Related to: 10