Work

Controlled Formation of Peptide Bonds in the Gas Phase

Year: 2011

Type: article

Abstract: Photoexcitation (using 157 nm vacuum ultraviolet radiation) of proton-bound peptide complexes leads to water elimination and the formation of longer amino acid chains. Thus, it appears that proton-bound dimers are long-lived intermediates along the pathway to peptide formation. Product specificity can be controlled by selection of specific complexes and the incorporation of blocking groups at the N- or C-termini. The product peptide sequences are confirmed using collision-induced dissociation.

Source: Journal of the American Chemical Society

DOI: 10.1021/ja205471n

Authors: Sunyoung Lee, Stephen J. Valentine, James P. Reilly, David E. Clemmer

Institutions: Indiana University Bloomington, Indiana University

Language: English

Topic: Mass Spectrometry Techniques and Applications

Subfield: Spectroscopy

Field: Chemistry

Domain: Physical Sciences

Open Access status: green

Funders: National Institutes of Health, National Institute of General Medical Sciences

Award: New Ion Mobility/Photodissociation Techniques for Analyzing Glycans