Nanosecond microscopy with spectroscopic resolution
Year: 2006
Type: article
Abstract: We demonstrate coherent anti-Stokes Raman scattering (CARS) microscopy in a wide-field setup with nanosecond laser pulse excitation. In contrast to confocal setups, the image of a sample can be recorded with a single pair of excitation pulses. For this purpose the excitation geometry is specially designed in order to satisfy the phase matching condition over the whole sample area. The spectral, temporal and spatial sensitivity of the method is demonstrated by imaging test samples, i.e. oil vesicles in sunflower seeds, on a nanosecond timescale. The method provides snapshot imaging in 3 nanoseconds with a spectral resolution of 25 wavenumbers (cm$^{-1}$).
Source: New Journal of Physics
Authors: Christoph Heinrich, Stefan Bernet, Monika Ritsch-Marte
Institution: Innsbruck Medical University
Language: English
Subfield: Biophysics
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $2,610
OpenAlex ID: W2068797016