Work

Arg-Pro-X-Ser/Thr Is a Consensus Phosphoacceptor Sequence for the Meiosis-Specific Ime2 Protein Kinase in Saccharomyces cerevisiae

Year: 2006

Type: article

Abstract: Ime2 is a meiosis-specific protein kinase in Saccharomyces cerevisiae that is functionally related to cyclin-dependent kinase. Although Ime2 regulates multiple steps in meiosis, only a few of its substrates have been identified. Here we show that Ime2 phosphorylates Sum1, a repressor of meiotic gene transcription, on Thr-306. Ime2 protein kinase assays with Sum1 mutants and synthetic peptides define a consensus Arg-Pro-X-Ser/Thr motif that is required for efficient phosphorylation by Ime2. The carboxyl residue adjacent to the phosphoacceptor (+1 position) also influences the efficiency of Ime2 phosphorylation with alanine being a preferred residue. This information has predictive value in identifying new potential Ime2 targets as shown by the ability of Ime2 to phosphorylate Sgs1 and Gip1 in vitro and could be important in differentiating mitotic and meiotic regulatory pathways.

Source: Biochemistry

DOI: 10.1021/bi061858p

Authors: Michael Moore, Marcus E. Shin, Adrian Bruning, Karen Schindler, Andrew Vershon, Edward Winter

Institutions: Rutgers, The State University of New Jersey, Thomas Jefferson University

Language: English

Topic: Fungal and yeast genetics research

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: National Institute of General Medical Sciences, National Institute of Diabetes and Digestive and Kidney Diseases

Awards: TRANSCRIPTIONAL REGULATION OF MEIOTIC GENES IN YEAST, G367406075, G424113996, Training Program in Biomolecular Signal Transduction, PROTEIN KINASE NETWORKS AND SPORULATION IN S. CEREVISIAE